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. 2017 Jun 29;9(1):177–189. doi: 10.1016/j.stemcr.2017.05.035

Figure 6.

Figure 6

hNSCs Migrated over the Cathode Electrode and to the SVZ, which Appeared to Have the Ability to Differentiate

(A) EF stimulation reversed the migration of hNSCs to the SVZ. hNSCs resided in large area of the SVZ 3 weeks post EF stimulation. EGFP signals are mainly detected in the SVZ. The cells in green are not co-labeled red for either GFAP or TUJ1, indicating no differentiation to astrocyte or neuron of the graft cells.

(B) Four months after transplantation and electrical stimulation, hNSCs maintained EGFP. Co-localization of EGFP signals with NEUN (in CA1/CA2 region of hippocampus), GFAP (mainly in OB), IBA1 (in hippocampus), or MBP (found in RMS) (red) suggests possible differentiation of hNSCs to neuron, astrocyte, microglia, or oligodendrocyte, respectively.

All nuclei are labeled in blue with DAPI. White arrows show typical differentiated cells. hNSCs, human neural stem cells; SVZ, subventricular zone; EF, electric field; OB, olfactory bulb; RMS, rostral migration stream. Scale bars, 100 μm (A) and 50 μm (B).