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. 2017 Jul 14;7:5375. doi: 10.1038/s41598-017-05039-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Production of TIMP-1 mutants. (a) Schematic representation of T1-WT, T1-F12A and T1-K47A proteins. (b) A representative western blot with the indicated antibodies of cell extracts and conditioned medium (cond. med) of stably transfected CHO cells with p3X-FLAG-CMV-14 expressing T1-WT, T1-F12A or T1-K47A. The relative protein level based on three independent experiments is indicated under each band. The gels were run under the same experimental conditions and were shown as cropped gels/blots (Cropped gels/blots are shown in Supplementary Figure S1).