Table 2.

Surface plasmon resonance data for direct binding of TIMP-1 mutants to immobilised LRP-1 domains II (DII) and IV (DIV).

	k on (0.106 M−1s−1)	k off (0.10−2 s−1)	K D (nM)
DII
T1-WT	1.28 ± 0.35	1.36 ± 0.20	10.8 ± 2.1
T1-F12A	1.07 ± 0.25	1.92 ± 0.18	18.5 ± 4.6
T1-K47A	0.70 ± 0.44	8.30 ± 0.78	10.4 ± 4.5
DIV
T1-WT	0.148 ± 0.001	0.56 ± 0.020	37.4 ± 1.7
T1-F12A	0.143 ± 0.006	0.55 ± 0.039	38.7 ± 3.8
T1-K47A	0.427 ± 0.300	1.14 ± 0.830	26.5 ± 0.9

Data are based on three measurements using five different concentrations for each measurement.

The equilibrium constants of dissociation (K _D) were calculated from the association (k _on) and dissociation (k _off) rate constants.

Mean values ± standard error are presented.