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. 2017 Jul 14;7:5466. doi: 10.1038/s41598-017-05770-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Total peripheral WBC count and peripheral WBC suspension staining 24 h after SBI. (A) Total peripheral WBC count was increased in VPC + SBI group compared to Sham and SPC + SBI groups. *p < 0.05 vs Sham, ^∆p < 0.05 vs SPC + SBI. Data are shown as mean ± SD. n = 8/group. (B) Hematoxylin and eosin (H&E) staining (n = 3/group) (C) Wrights staining (n = 3/group) and (D) DAPI staining (n = 3/group) of peripheral WBC suspension showing segmented granulocytes in VPC + SBI group. All scale bars = 25 μm.