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. 2017 Jul 14;7:5466. doi: 10.1038/s41598-017-05770-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence staining and western blot showing inflammatory markers in the right frontal peri-resection site in Sham, SPC + SBI and VPC + SBI groups 24 h after SBI. (A) Immunofluorescence staining showed fewer CD68- and MPO-positive cells in VPC + SBI rats compared to SPC + SBI group. n = 2/group. Scale bar = 50 μm. (B) Western blot quantification showed that neutrophil elastase was reduced in VPC + SBI group compared to SPC + SBI group. *p < 0.05 vs Sham, ^∆p < 0.05 vs SPC + SBI. Data are shown as mean ± SD. n = 6/group. The full length western blot pictures are shown in Supplemental Figure S3.