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. 2017 Jul 14;7:5474. doi: 10.1038/s41598-017-05777-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry detection of surface markers of human platelet free blood plasma extracellular vesicles (EVs) conjugated onto latex beads. EVs were isolated either by Total Exosome isolation kit (Life Technologies, by Thermo Fisher Scientific, Waltham, MA, USA) (empty histograms with dotted lines) or ultracentrifugation at 100,000 g (empty histograms with continuous lines) from three different human plasma samples, respectively. Antibody binding to BSA-coated latex beads is shown in gray histograms.