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. 2017 Jul 14;7:5488. doi: 10.1038/s41598-017-05945-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of terminal sugar residues of glycoproteins in symbiotic and free-living Symbiodinium. (A) Six proteins (two Symbiodinium proteins and four control glycoproteins) were analyzed by SDS-PAGE and stained with SYPRO^® Ruby. (B) Western blotting was performed using the 2–6F mAb. Five types of lectin-conjugated alkaline phosphatase, (C) GNA, (D) PNA, (E) MAA, (F) SNA, and (G) DSA, were used to detect different glycans. M: marker; Sym: total proteins of symbiotic Symbiodinium (clade C) freshly isolated from E. glabrescens; Cul: total proteins of cultured Symbiodinium CCMP 2466 (clade C); CPY: carboxypeptidase Y; Tf: transferrin; Fet: fetuin; AF: asialofetuin. Four control glycoproteins were used: CPY, Tf, Fet, and AF. For GNA, CPY is a positive control, and Tf is a negative control. For PNA, AF is a positive control, and CPY is a negative control. For MAA, CPY is a positive control, and Fet is a negative control. For SNA, Tf is a positive control, and CPY is a negative control. For DSA, Fet is a positive control, and CPY is a negative control.