. 2017 Jul 14;7:5460. doi: 10.1038/s41598-017-05539-y

Table 2.

Distribution constants K _D of probes for L_o microdomains and implications for FRET in the presence of L_d or L_o domains.

Donor	K _D for L_o micro domains^*	Acceptor	K _D for L_o micro domains^*	Implications for FRET in the presence of L_d nanodomains	Implications for FRET in the presence of L_o nanodomains
g-GM₁	2.5 ± 0.28	r-GM₁	1.6 ± 0.63	Increased^,*	Slightly increased^,*
CF-PEG-DSPE	1.5 ± 0.54	Rh-PEG-DSPE	3.5 ± 0.90	Slightly increased^,*	Slightly increased^,*
g-GM₁	2.5 ± 0.28	DiD	0.1 ± 0.01	No change^**	Decreased^**
Atto 488-DOPE	0.29 ± 0.115	Atto 633-DOPE	0.03 ± 0.013	No change^**	Increased^**

Note that K _D for L_d nanodomains are shown in Table 1. *Determined by intensity measurements (see SI) in L_d/L_o phase separated bilayers of DOPC/Chol/SM (55/25/20); ^**as compared to FRET obtained in a homogeneous bilayer; ^***this conclusion is drawn based on K _Ds given in Table 1.