Figure 4.

Growth and survival of Chlamydomonas cells subjected to various nutritional deprivation conditions. Cells grown to logarithmic phase in TAP medium were serially diluted in water, 5 μl-aliquots spotted on plates of the appropriate media and incubated for 7 to 15 days under continuous illumination. Maa7-IR44s, CC-124 strain containing a transgene expressing FLAG-tagged AGO3; Mut-20, TSN1 deletion mutant, in the Maa7-IR44s background, defective in sRNA biogenesis¹⁴; Gluc(1x), wild type strain derived from CC-124; ago3-1, AGO3 disrupted mutant, in the Gluc(1x) background, defective in RNA interference²⁸. (A) Cells grown under mixotrophic conditions in the presence or absence of sulfur. (B) Cells grown under photoautotrophic conditions in the presence or absence of phosphorus. (C) Cells grown under mixotrophic conditions in the presence or absence of nitrogen. (D) Cells grown under photoautotrophic conditions in the presence or absence of nitrogen.