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. 2017 Jul 14;7:5462. doi: 10.1038/s41598-017-05561-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the number of predicted cleavage targets for lowly expressed and highly expressed miRNAs. Boxplots show the number of predicted cleavage targets for miRNAs with low expression (<500 CPM) and high expression (≥500 CPM) identified in the AGO3-pulldown libraries from C. reinhardtii (blue) and for species-specific or conserved miRNAs from Arabidopsis thaliana (red) or Arabidopsis lyrata (green). Conserved miRNAs are shared between A. thaliana and A. lyrata and many have experimentally validated roles. MicroRNAs that had no predicted targets were excluded from this plot. The average number of targets (including outliers) and the sample size for each category are shown above the boxes. The target numbers were compared between the two expression-level groups for each species and an asterisk next to the species abbreviation indicates a significant difference (p < 0.05 by Wilcoxon rank sum test).