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. 2017 Jul 14;7:5490. doi: 10.1038/s41598-017-06122-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro cytotoxicity experiments. (a) Relative viability of MCF-7 cells incubated with different concentrations of Fe₃O₄/ICG@PLGA/PFP NPs under laser irradiation (808 nm, 1 W/cm², 5 min). (b) Fluorescence images of Hoechst 33342/PI co-stained MCF-7 cells incubated with Fe₃O₄/ICG@PLGA/PFP NPs (0.5 mg/mL) and exposed to the 808 nm laser irradiation (1 W/cm², 5 min). Live and dead cells were stained with Hoechst 33342 (blue color) and PI (red color), respectively.