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. 2017 Jul 14;7:5410. doi: 10.1038/s41598-017-05595-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HMGCR is over-expressed in ovarian cancer cells and regulated by TP53. (a) The expression of HMGCR was measured by immunoblotting and quantified (b) in a panel of ovarian cancer cells and compared to non-cancerous cells including normal human ovarian epithelia (HOE), human foreskin fibroblasts (HFF) normal lung epithelia (NL20). (c) Wild (WT) and TP53 variants were over-expressed in SkOv-3 cells and p53 and HMGCR measured by immunoblotting. The numbers above the blots show the mean change in protein normalized to GAPDH (n = 3 lysates) and expressed as ratio of that measured in cells transfected with the vector. D. Ovcar-3 cells were transfected with non-targeting (NT) siRNA or 4 different p53 siRNA (#1, #2, #3, #4) and reduction in p53 and HMGCR protein confirmed by immunoblotting (n = 3 independent transfections). Expression was quantified and normalized to that in normal cells (mean ± S.D, n = 3, *paired t-test, P < 0.05, compared to cells transfected with vector). HMGCR mRNA was measured by QPCR, normalized to GAPDH expression and expression was calculated as a fraction of that measured in cells transfected with NT siRNA (paired t-test, n = 3 independent transfections; *P < 0.05; **P < 0.005 compared to cells transfected with NT siRNA). Images presented in this panel were cropped to remove surrounding white space.