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. 2017 Jul 14;7:5430. doi: 10.1038/s41598-017-05648-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of a zebrafish reporter ubiquitously expressing mCherry-MBD. (a) The structure of human MBD1, a comparison of the amino acid sequences of human and zebrafish methyl-binding domains, and the Tol2-based vector structure including promoter, the mCherry-MBD probe, and IRES-nlsEGFP. MBD, methyl binding domain; nls, nuclear localization signal; CXXC, cysteine-rich domains; TRD, transcriptional repression domain. (b) Fluorescence images of Tg(bactin2:mCherry-MBD-IRES-nlsEGFP) F1 embryos at 6, 10, and 24 hpf. Magnified images in white boxes display the mCherry-MBD channel (left) and IRES-nlsEGFP channel (right). (c) The distribution of mCherry-MBD in the adult zebrafish heart, brain, liver, ovary, and gut. The reporter is labeled as zebraRDM.