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. 2017 Jul 14;7:5430. doi: 10.1038/s41598-017-05648-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Colocalization of mCherry-MBD with heterochromatin and modified histones. (a) The correlation between Hoechst staining and the distribution patterns of the probes in 5 hpf embryos. Higher magnification images are arranged below. The orange boxes denote cells in mitotic metaphase; note that the nlsEGFP signal has disappeared due to nuclear envelope breakdown. White boxes below show magnified images. White arrowheads label puncta in Hoechst-stained nuclei. (b) Columns represent the overlap and Pearson coefficients for colocalization between Hoechst and the probes. P values < 0.0001, N = 27. (c) The colocalization pattern of mCherry-MBD and H3K9me3/ac. Lower rows showed subcelluar structure in one single cell nuclei. (d) Time lapse imaging of mCherry-MBD dynamics during the cell cycle. (e) The distribution of mCherry-MBD in phospho-Histone H3-positive metaphase cells. (f) The linear regression analysis between the intensity of MBD and that of modified histones. The plots represent cells or ROIs (region of interest) selected by image j (H3K9me3, N = 66 ROIs; H3K9ac, N = 112 ROIs; PH3, N = 10 cells). The intensity value ranged from 0 to 255. P value < 0.0001.