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. 2017 Jul 14;7:5430. doi: 10.1038/s41598-017-05648-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential methylated cells and gene loci recognized by the probe mCherry-MBD. (a) The mCherry fluorescence dot plot of cells in zebraRDM. R2, MBD+ group; R4, MBD− group. (b) Cell numbers and proportion of sorted cells. (c) Columns represent the percentage of 5 mC in DNA isolated from the MBD+ and MBD− groups (P = 0.026). (d) The GFP fluorescence dot plot of Wnt-responsive cells. (e) Columns represent the percentage of 5 mC in DNA isolated from TCF+ and TCF− groups (P = 0.0489). (f) The Chip-qPCR results of three genes′ hypo-/hyper-methylated sites. The images above are schematic pictures of three genes’ methylation status and their hypo-/hyper-methylated sites’ position. Columns represent the content of specific sites’ DNA fragment, normalized to the AB control. TSS, transcription start site; Hypo-sites, hypo-methylated sites; Hyper-sites, hyper-methylated sites.