Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 14;7:5393. doi: 10.1038/s41598-017-05821-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Concept of sPRE and exemplary sPRE data of the UUCG tetraloop. (a) sPRE data provide a quantitative measure for solvent accessibility and encode distance-to-surface information. sPRE data are acquired by titrating the RNA with the paramagnetic compound Gd(DTPA-BMA). (b) The NMR solution structure of the UUCG tetraloop (PDB code 2KOC) is shown and for illustration purpose, two solvent-exposed protons (blue spheres) and two buried protons (orange spheres) are highlighted. (c) Schematic representation of the UUCG tetraloop (top) and the GTP-bound GTP aptamer. (d) NMR spectra of the UUCG tetraloop are shown in the absence (black) and presence (magenta) of Gd(DTPA-BMA) with circles around the peaks corresponding to the protons shown in (b). (e) Quantitative sPRE data are obtained by measuring the longitudinal proton R ₁ relaxation rate as a function of the concentration of Gd(DTPA-BMA). (f) Proton R ₁ rates increase linearly with the concentration of the paramagnetic compound and the slopes correspond to the sPRE values of the respective resonances highlighted in (b).