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. 2017 Jul 14;7:5393. doi: 10.1038/s41598-017-05821-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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sPRE data correlates well with RNA structure. (a) Experimental sPRE data (black) are compared to predicted sPRE data based on NMR solution structures of the UUCG tetraloop (2KOC) and the GTP-bound aptamer (5LWJ). Data are shown for sugar protons (H1′) and aromatic protons (H6 and H8) as indicated. (b) NMR solution structures of the UUCG tetraloop^{37, 38} (2KOC, left) and the GTP-bound aptamer^{39, 40} (5LWJ, right, heavy atoms of GTP ligand shown in green) are shown. All protons for which a sPRE value was obtained are shown as spheres and colored according to the sPRE (blue corresponding to high and orange corresponding to low sPRE values).