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. 2017 Jul 13;8:16091. doi: 10.1038/ncomms16091

Figure 5. Preferential electrical coupling depends on a low density of interneuron labelling.

Figure 5

(a) 3D reconstruction of consecutive neocortical sections infected with a mixture of serially diluted, low-titre RCAS expressing EGFP or mCherry. Note the clear spatial segregation of EGFP- and mCherry-expressing interneuron clusters in the neocortex. (b) NND analysis of labelled interneurons in the neocortices infected with a mixture of low-titre RCAS expressing EGFP or mCherry (n=13 hemispheres). Data are presented as mean±s.e.m. ***P<0.001 (Kolmogorov–Smirnov test). (c) 3D reconstruction of consecutive neocortical sections infected with a mixture of high-titre RCAS expressing EGFP or mCherry. Note no clear spatial segregation of EGFP- and mCherry-expressing interneuron clusters in the neocortex. (d) NND analysis of labelled interneurons in the neocortices infected with a mixture of high-titre RCAS expressing EGFP or mCherry (n=4 hemispheres). Data are presented as mean±s.e.m. NS, not significant (Kolmogorov–Smirnov test). (e,f) Summary of the frequencies of (e) electrical and (f) chemical synaptic connectivity among EGFP-expressing interneuron clusters labelled at a low or high density, as well as among non-clonally related EGFP- and mCherry-expressing interneuron clusters after P7. ***P<0.001; NS, not significant (χ2 test).