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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: Nat Genet. 2016 Dec 19;49(2):193–203. doi: 10.1038/ng.3741

Figure 1. A pooled, genome-wide CRISPR screen for HIV host dependency factors (HDFs).

Figure 1

(a) Outline of genome-wide CRISPR screen strategy.

(b) Flow cytometry of cells infected with the HIV-1 strain JR-CSF and expressing GFP as a reporter of productive HIV infection. Where indicated, cells are transduced with sgCCR5 or an sgRNA that does not target protein-coding sequences in the human genome (non-targeting control).

(c) Flow cytometry of CD4 and CCR5 surface expression on WT GXRCas9 cells and GXRCas9 cells transduced with the genome-wide sgRNA library and serially infected with the HIV-1 strain JR-CSF.

(d) Log2-fold change in abundance of the 5th most enriched sgRNA for every gene following HIV infection. See also Supplementary Table 1 and Supplementary Fig. 1.

(e) Enrichment of individual sgRNAs for three candidate HDFs and two control genes. Values indicate log2-fold change in abundance following HIV infection. Uninfected values are from GXRCas9 cells transduced with the genome-wide sgRNA library and cultured for 3 weeks.