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. Author manuscript; available in PMC: 2017 Aug 22.
Published in final edited form as: Dev Cell. 2016 Aug 11;38(4):413–429. doi: 10.1016/j.devcel.2016.07.012

Figure 3. nrg-7 is required for neoblast repopulation after sublethal irradiation.

Figure 3

(A) WISH of putative egf ligands in unirradiated and 6,000 rads irradiated (1 dpi) planarians. (B) FISH for smedwi-1 in control(RNAi) and nrg-7(RNAi) planarians at 7 and 14 dpi. (C) Quantification of smedwi-1+ cells/mm2 in nrg-7(RNAi) animals. *, p<0.05. (D) Immunostaining with anti-H3P antibody in control(RNAi) and nrg-7(RNAi) planarians at 7 and 14 dpi. (E) Quantification of H3P+ cells/mm2 in nrg-7(RNAi) planarians. (F) FISH for smedwi-1 in control(RNAi) and nrg-7(RNAi) unirradiated planarians at 14 days post feeding. (G) Immunostaining with anti-H3P antibody in control(RNAi) and nrg-7(RNAi) unirradiated planarians. (H) Quantification of H3P+ cells/mm2 in nrg-7(RNAi) compared to controls. (I) Purified recombinant MYC-NRG-7-HIS (NRG-7) and the extracellular domain of FLAG-EGFR-3-HIS (EGFR-3) (residues 1 to 818 aa) were incubated together and NRG-7 was captured using anti-MYC affinity resin. EGFR-3 and NRG-7 are detected only in the experimental reaction containing NRG-7. Scale bars= 200 μm (A, B, D), 500 μm (F, G). See also Figure S4.