Figure 3. Cancer stem cells surface markers and ALDH activity of MDA-MB-231 spheroids assessed by flow cytometry analysis.

(A) Spheroid cells exhibited a significantly higher percentage of CD44+/CD24− cells populations (70.42 ± 2.22%) compared to that of the parental cells (27.28 ± 1.65%). (B) ALDH activity also exhibited an increased in spheroid cells with 29.43 ± 1.07% compared to parental cells 1.64 ± 0.52%. (C) Representative flow cytometric dot plots were shown whereby R2 is the region of ALDH-positive cells.