NHE3loxloxCre compared to Con fed a control diet (0.8% NaCl) show comparable body weight, plasma concentrations of Na+, K+, aldosterone, plasma osmolality and blood pH. Fluid intake and urine osmolality were significantly higher and lower, respectively, in NHE3loxloxCre mice. Urinary pH was significantly higher in NHE3loxloxCre mice. GFR was significantly lower, while Na+ intake and urinary Na+ excretion tended to be higher in NHE3loxloxCre compared to Con mice. Baseline fluid and food intake n = 31–32; for other parameters n = 9–32. *P < 0.05 versus Con.