Figure 11. Lower abundance of the Cl⁻/bicarbonate exchanger (pendrin) and higher abundance of H⁺-ATPase β1-subunit expression in NHE3^loxloxCre mice.

Expression of the Na⁺/K⁺-ATPase α-subunit was unaffected by dietary NaCl or genotype. In NHE3^loxloxCre mice, pendrin levels were significantly lower compared to Con mice but unaffected by dietary NaCl intake. Vice versa, the H⁺-ATPase β1-subunit levels were significantly higher in NHE3^loxloxCre compared to Con mice, but also unaffected by dietary NaCl. Aquaporin-2 (AQP2) expression was significantly greater in NHE3^loxloxCre on high compared to low NaCl intake, an effect absent in Con mice. Expression of phosphorylated AQP2 at serine 256 (pS256 AQP2) was not affected by genotype or dietary NaCl. *P < 0.05 versus high NaCl same genotype, ^#P < 0.05 versus Con same diet.