Table 1.

Primer sequences used for PCR amplification assays

Primer pairs	Target sequence 5′–3′	Target gene	Tm	Amplicon size	Specificity	Source
16S-F 16S-R	AGAGTTTGATCCTGGCTCAG ACGGCTACCTTGTTACGACTT	16-S ribosomal DNA (16 S rDNA)	58 °C	~1500 bp	Bacterial strains	Turner et al. (1999)
ITS-1 ITS-4	TCCGTAGGTGAACCTGCGG TCCTCCGCTTATTGATATGC	Internal transcribed spacer (ITS)	58 °C	~550 bp	Fungal isolates	White et al. (1990)
HGF-F HGF-R	GCCTCCCCAACCATTCCCTTA TCACGGATTTCTGTTGTGTTTC	Human growth factor (HGF)	60 °C	~430 bp	Human tissues	Tomida and Saito (2004)
NAD-F NAD-R	ATCACCGAACCTGCACTCAGGAA TAGCCCGACCGTAGTGATGTTAA	Nicotinamide adenine dinucleotide (NAD)	68 °C	~800 bp	Plant species	Mannerlöf and Tenning (1997)