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Purification steps of XynAMG1 and zymogram analysis. Proteins were separated with 12% gel SDS-PAGE. Lane (Zym) zymogram, lane (C): total soluble cell lysate from E. coli host expressing XynAMG1, lane (Co): Trx-His-XynAMG1 fusion protein purified by TALON cobalt resin, lane (E): purified XynAMG1 after digestion with enterokinase, and lane (M): protein molecular weight markers.
