Table 1.

Primers Used for Site-Directed Mutagenesis of tLRAT

Q177S	(+): 5′-CCG ATC AGT CCC TCG TCC GAC AAG T-3′
	(−): 5′-A CTT GTC GGA CGA GGG ACT GAT CGG-3′
Q177E	(+): 5′-CCG ATC AGT CCC GAG TCC GAC AAG T-3′
	(−): 5′-A CTT GTC GGA CTC GGG ACT GAT CGG-3′
D49N	(+): 5′-C CAC CGA GGC AAC GTG CTG GAG GTG-3′
	(−): 5′-CAC CTC CAG CAC GTT GCC TCG GTG G-3′
D67A	(+): 5′-C ATC TAC CTA GGA GCC AAC CGT GTT GC-3′
	(−): 5′-GC AAC ACG GTT GGC TCC TAG GTA GAT G-3′
D76N	(+): 5′-CAC ATG ATG CCC AAC ATC CTG TTG GCC-3′
	(−): 5′-GGC CAA CAG GAT GTT GGG CAT CAT GTG-3′
D111N	(+): 5′-GC ATC CGC GTG AAC ACA GTG GAG GAC-3′
	(−): 5′-GTC CTC CAC TGT GTT CAC GCG GA T GC-3′
D128N	(+): 5′-GTC AAT CAC CTG AAC GAG TCC CTC CAG-3′
	(−): 5′-CTG GAG GGA C TC GTT CAG GTG AT T GAC-3′
D191N	(+): 5′-G ATA ATT ATT CGT AAT CAG AGA AGT GTT CTC-3′
	(−): 5′-C GAG AAC ACT TCT CTG ATT ACG AAT AA T TAT CG-3′