FIGURE 2.

CyTOF measurements on cisPt-live/cisPt+dead cell discrimination and the number of silver containing cells in a population. P. putida KT2440 cells were cultivated in M12 medium with AgNP-10 (1.29 mg/L), with AgNO₃ (0.19 mg/L) as positive control, and untreated as negative control. Growth curves were shown for 72 h by measuring OD₆₀₀. Cells were harvested at 12, 48, 72 h and measured by CyTOF. Light gray bars showed cells without silver load, while dark gray bars showed silver containing cells. Standard errors were from replicate analysis.