Figure 4.

Effect of shRNA C1GT suppression on antibody accessibility to cell surface anoikis-initiating molecules. The shRNA C1GT transfected or shRNA control cells from the MUC1- positive F3 cells were incubated with antibodies against E-cadherin, integrinβ1, CD44 or Fas. After washing and application of FITC-conjugated secondary antibody, the cells were analysed by flow cytometry (a, solid and dotted lines show the peak positions of control and shRNA C1GT transfected cells, respectively. Dotted line shifted to the left or right of the solid line reveals reduction or increase of molecule expression). Mean florescence intensity of the antibody binding are shown in b. Cells were also lysed and analysed by immunoblotting (c) for the expression of these molecules with the same antibodies as in a. **P<0.01, ***P<0.001.