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. 2017 Jul 5;37(27):6442–6459. doi: 10.1523/JNEUROSCI.3520-16.2017

Figure 6.

Figure 6.

Depletion of βIII spectrin results in formation of aberrant synapses. A, B, Fluorescence staining of 17 DIV neurons transfected with control shRNA (shc) or βIII spectrin shRNA (sh#2) with phalloidin, axonal marker SMI312, and synapsin I antibody. Scale bar, 10 μm. B, High-magnification images showing spiny synapses in a control cell (top) and shaft synapses in a βIII spectrin-depleted cell (bottom). Scale bar, 5 μm. C, Average numbers of synapsin I puncta per 100 μm of axon length (shc: N = 664 puncta in 9 cells; sh#1: N = 631puncta in 10 cells; sh#2: N = 615 puncta in 10 cells). Error bars indicate SD. D, Staining of PSD95 and synapsin I in control (shc) and βIII spectrin-depleted (sh#2) cells. Scale bar, 5 μm. Boxed areas are zoomed at right. E, Quantification of synapsin I puncta associated with “bright” or “weak” PSD95 puncta or not associating with PSD95 puncta (“synapsin I only”) in control and βIII spectrin-depleted cells (shc, N = 694 puncta in 5 cells; sh#2, N = 578 puncta in 5 cells).