Table 2.
Combination of antibodies used to characterize natural killer cell phenotype.
| Tube # | Antibody | Source |
|---|---|---|
| 1 | IgG1 isotype control | Biolegend |
| IgG2a isotype control | Biolegend | |
| IgG2b isotype control | Biolegend | |
| PE-conjugated goat anti-mouse IgG1 | Southern Biotech | |
| PE-conjugated goat anti-mouse IgG2a | Southern Biotech | |
| PE/Cy7-conjugated goat anti-mouse IgG2b | Southern Biotech | |
| FITC-conjugated anti-CD3 | Beckman Coulter | |
| PC5-conjugated anti-CD56 | Beckman Coulter | |
| FITC-conjugated anti-CD14 | Beckman Coulter | |
| FITC-conjugated anti-CD20 | Biolegend | |
| APC/Cy7-conjugated IgG1 | Biolegend | |
| BV510-conjugated IgG1 | Biolegend | |
| BV421-conjugated mouse IgM | Becton-Dickinson | |
| 2 | Anti-NKG2A (Z199) [IgG2b] | A. Moretta, S. Parolini |
| Anti-KIR2 DL1/S1 (AZ115) [IgG1] | A. Moretta, S. Parolini | |
| Anti-KIR2 DL2/S2 (GL183) [IgG1] | A. Moretta, S. Parolini | |
| Anti-KIR3D (AZ158) [IgG2a] | A. Moretta, S. Parolini | |
| PE-conjugated goat-anti-mouse IgG1 | Southern Biotech | |
| PE-conjugated goat anti-mouse IgG2a | Southern Biotech | |
| PE/Cy7-conjugated goat anti-mouse IgG2b | Southern Biotech | |
| FITC-conjugated anti-CD3 | Beckman Coulter | |
| PC5-conjugated anti-CD56 | Beckman Coulter | |
| FITC-conjugated anti-CD14 | Beckman Coulter | |
| FITC-conjugated anti-CD20 | Biolegend | |
| APC/Cy7-conjugated anti-CD16 | Biolegend | |
| BV510-conjugated anti-CD69 | Biolegend | |
| BV421-conjugated anti-CD57 | Becton-Dickinson | |
| 3 | Anti-SIGLEC7 (Z176) [IgG2b] | A. Moretta, S. Parolini |
| Anti-LIR1 (F278) [IgG1] | A. Moretta, S. Parolini | |
| PE-conjugated goat-anti-mouse IgG1 | Southern Biotech | |
| PE/Cy7-conjugated goat anti-mouse IgG2b | Southern Biotech | |
| FITC-conjugated anti-CD3 | Beckman Coulter | |
| PC5-conjugated anti-CD56 | Beckman Coulter | |
| FITC-conjugated anti-CD14 | Beckman Coulter | |
| FITC-conjugated anti-CD20 | Biolegend | |
| APC/Cy7-conjugated anti-CD16 | Biolegend | |
| BV510-conjugated anti-CD69 | Biolegend | |
| BV421-conjugated anti-CD57 | Becton-Dickinson | |
| 4 | Anti-NKG2C [IgG2b] | R&D Systems |
| Anti-KIR2 DL1/S1 (11PB6) [IgG1] | A. Moretta, S. Parolini | |
| Anti-KIR2 DL2/S2 (GL183) [IgG1] | A. Moretta, S. Parolini | |
| Anti-KIR3D (AZ158) [IgG2a] | A. Moretta, S. Parolini | |
| PE-conjugated goat-anti-mouse IgG1 | Southern Biotech | |
| PE-conjugated goat anti-mouse IgG2a | Southern Biotech | |
| PE/Cy7-conjugated goat anti-mouse IgG2b | Southern Biotech | |
| FITC-conjugated anti-CD3 | Beckman Coulter | |
| PC5-conjugated anti-CD56 | Beckman Coulter | |
| FITC-conjugated anti-CD14 | Beckman Coulter | |
| FITC-conjugated anti-CD20 | Biolegend | |
| APC/Cy7-conjugated anti-CD16 | Biolegend | |
| BV510-conjugated anti-CD69 | Biolegend | |
| BV421-conjugated anti-CD57 | Becton-Dickinson | |
| 5 | Anti-CXCR1 [IgG1] | Santa Cruz |
| PE-conjugated goat-anti-mouse IgG1 | Southern Biotech | |
| FITC-conjugated anti-CD3 | Beckman Coulter | |
| PC5-conjugated anti-CD56 | Beckman Coulter | |
| FITC-conjugated anti-CD14 | Beckman Coulter | |
| FITC-conjugated anti-CD20 | Biolegend | |
| APC/Cy7-conjugated anti-CD16 | Biolegend | |
| Pacific Blue-conjugated anti-CCR7 | Biolegend |