Figure 5.
Disappearance of the sulfide-induced CT complex of ndSQR in the presence of GSH. A, ndSQR (40 μm) in 100 mm potassium phosphate buffer, pH 7.4, was rapidly mixed 1:1 (v/v) with Na2S (80 μm) and aged for ∼35 ms to form the CT complex, after which the mixture was rapidly mixed 1:1 (v/v) with 100 mm potassium phosphate buffer, pH 7.4 ± GSH (50 mm). The absorbance at 675 nm was monitored over a period of 3 s. B, spectral changes accompanying the disappearance of the CT complex in the presence of 25 mm GSH, monitored over 3 s, which demonstrated reduction of FAD (thick black line). C, dependence of the kobs for the disappearance of the CT complex in ndSQR on the GSH concentration. The data represent the mean ± S.D. of two independent experiments.