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Kinetics of FAD oxidation in ndSQR by coenzyme Q1. A, ndSQR containing reduced FAD (20 μm) was produced via photoreduction as described under “Experimental Procedures” followed by reaction by 1:1 (v/v) rapid mixing with CoQ1 (40 μm) for 5 s, which resulted in the oxidation of FAD (thick black line). B, dependence of the kobs for ndSQR-mediated CoQ1 reduction on the concentration of CoQ1. The data represent the mean ± S.D. of two independent experiments.
