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PAGE assays for RAD52 ssDNA annealing activity. A and B, gel image of an ssDNA annealing by RAD52 and GFP-RAD52 in the absence of RPA (A) and corresponding quantitation analysis (B). C and D, ssDNA annealing by RAD52 and GFP-RAD52 in the presence of RPA (C) and corresponding quantitation analysis (D). Error bars represent standard deviation from three experiments.
