Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 27;292(28):11702–11713. doi: 10.1074/jbc.M117.794545

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 3. — RAD52-binding behavior. A, kymograph showing real time binding of GFP-RAD52 (50 pm; green) to an RPA-RFP-coated ssDNA molecule (magenta). B, kymograph showing binding of GFP-RAD52 (50 pm; green) to a wtRFP-coated ssDNA molecule (unlabeled). C, kymograph showing that GFP-RAD52 (50 pm; green) does not bind to a control surface within the sample chamber that lacks an ssDNA molecule. D, wide-field images of a single ssDNA molecule showing GFP-RAD52 complexes (green; highlighted with arrowheads), the RPA-RFP-coated ssDNA (magenta), and an overlaid image of the GFP-RAD52 and RFP-RFP signals. E, binding site distribution of GFP-RAD52 bound to the RPA-coated ssDNA molecules. Error bars represent standard deviation obtained through Bootstrap analysis of the data. F, histogram showing the intensity distribution of the RAD52-GFP complexes.