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. 2017 Jun 1;292(28):11740–11750. doi: 10.1074/jbc.M117.781369

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — Knockdown of BMP4 modulated androgen and estrogen levels. TCs and GCs were isolated from mice ovaries and treated with adenovirus to disrupt BMP4 expression. A, testosterone in TCs supernatant and estrogen in GCs supernatant were measured using chemiluminescence immunoassay. B, qPCR analysis of CYP11A, HSD3B2, CYP17A1, and BMP4 mRNA levels in TCs (left) and CYP19A1 and BMP4 mRNA levels in GCs (right) upon BMP4 knockdown. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with control group.