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. 2017 May 24;292(28):11751–11759. doi: 10.1074/jbc.M117.787341

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — Only Ptc6p dephosphorylates and activates Pda1p in vitro. A, immunoblots (IB) of phosphorylated Pda1p on Ser-313 (S313, left panels) or voltage-dependent anion channel (VDAC) (loading control, right panels). Mitochondrial lysate from Δptc6 was treated with recombinant Ptc5p, Ptc6p, Ptc7p, or Hfd1p. Samples from six different time points were taken and analyzed by immunoblotting. B, relative in vitro phosphatase activity against pNPP for recombinant Ptc5p, Ptc6p, Ptc7p, or Hfd1p (mean ± S.D., n = 3). The activity is normalized to total protein amount. C, relative PDC activity of mitochondrial lysates from Δptc6 yeast after 30 min of treatment with recombinant Ptc5p, Ptc6p, Ptc7p, or Hfd1p (mean ± S.D., n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001.