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. 2017 May 25;292(28):11777–11791. doi: 10.1074/jbc.M117.781104

Figure 5.

Figure 5.

MKL promotes Pfn expression through modulating STAT1 in HEK-293 cells. A and B, immunoblot analyses of Pfn expression in HEK-293 cells following either knockdown (A) or overexpression (B) of STAT variants (lysates were prepared 72 and 48 h after siRNA and plasmid transfections, respectively). C, immunoblot analyses of HEK-293 extracts showing the effect of STAT1 overexpression with or without STAT3 knockdown on Pfn expression. D, immunoblot analyses of HEK-293 extracts showing the effect of FLAG-MKL1 overexpression with or without STAT1 knockdown on Pfn expression (siRNA transfection was performed 24 h prior to plasmid transfection). All bar graphs accompanying the immunoblots show the mean ± S.D. values of the fold-changes in Pfn expression with respect to the corresponding control transfection condition (all data summarized from three experiments; *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, not significant; EV, empty vector). GAPDH blots serve as the loading control.