Table 1.

Comparison of kinetic parameters of ADS lyase from different organisms

Assay conditions were: C. neoformans (40 mm PPB, pH 8, 37 °C), S. cerevisiae (50 mm sodium phosphate buffer, pH 7.0, 35 °C), E. coli (50 mm HEPES buffer, pH 8.5, 25 °C), M. tuberculosis and M. smegmatis (succinic acid, sodium dihydrogen phosphate and glycine in ratio 2:7:7, pH 7.6, 37 °C), P. falciparum (50 mm PPB, pH 7.4, 25 °C), L. donovani (20 mm HEPES-KOH, pH 7, 25 °C), B. subtilis (50 mm HEPES, pH 7, 25 °C), H. sapiens (150 mm NaCl, pH 7, 25 °C). Values are shown ± S.E.

Species	Kcat	Km	Kcat/Km	References
	s−1	μm	μm−1s−1
C. neoformans	25.2	22.2 ± 2.9	1.1	This work
S. cerevisiae	NDa	12	ND	10
E. coli	ND	ND	16.5	17
M. tuberculosis	0.1 ± 0.0	204.2 ± 48.2	0.0005	76
M. smegmatis	0.7 ± 0.0	43.7 ± 2.6	0.02	76
P. falciparum	7.5 ± 0.7	32.0 ± 1.7	0.23	18
L. donovani	28.0	24.0	ND	40
B. subtilis	1.3 ± 0.2	3.5 ± 0.4	3.8 × 105	77
H. sapiens	97.0 ± 5.2	1.8 ± 0.3	53.9	39

^a ND denotes no data.