Figure 1.

Pin1 expressions are increased in islets from obese mice. A, islets were prepared from mice fed a normal diet or a high-fat diet for 20 weeks (n = 4). B, islets were isolated from 10-week-old male wild-type mice or ob/ob mice. Pin1 protein levels in the islets were demonstrated by immunoblot (IB) analysis (n = 4). C and D, glucose tolerance and insulin tolerance tests were performed, using three groups of mice (n = 5). E, islets were isolated from 8-week-old male mice. The islets were then incubated with HKR buffer containing 3 or 20 mm glucose for 1 h. F, islet areas were measured to allow comparisons among the three groups. **, p < 0.01; ***, p < 0.001; n.s., not significant. Representative data from three independent experiments are shown. Error bars, S.E.