Table 1.

Thermodynamic parameters derived from ITC

For all mutants, n = 3.

N-cTnC construct	N	Kd	ΔS	ΔH	ΔG
		μm	J·mol−1·deg−1	J·mol−1	kJ·mol−1
WT	1.05 ± 1e-2	14.9 ± 0.7	140.5 ± 2	1.43e4 ± 6e2	−2.76e4 ± 1e2
A8V	1.01 ± 2e-2	15.3 ± 0.9	141.6 ± 2	1.47e4 ± 4e2	−2.75e4 ± 2e2
L29Q	0.99 ± 1e-2	14.2 ± 0.4	145.0 ± 1	1.56e4 ± 4e2	−2.77e4 ± 5e1
A31S	0.88 ± 3e-2	11.8 ± 1.1	124.5 ± 2a	8.99e3 ± 3e2a	−2.81e4 ± 2e2
L48Q	1.02 ± 3e-2	1.48 ± 0.1a	50.1 ± 1a	−1.84e4 ± 2e2a	−3.33e4 ± 8e1a
Q50R	1.05 ± 1e-2	5.85 ± 0.1a	120.8 ± 1a	6.15e3 ± 1e2a	−2.99e4 ± 8e1a
C84Y	0.96 ± 2e-2	4.19 ± 0.9a	113.9 ± 2a	3.12e3 ± 1e2a	−3.09e4 ± 5e2a

^a Significant difference from WT (p < 0.05).