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Anti-FBG antibodies block the interaction between the mosquito peritrophic membrane and recombinant FREP1 protein. 18 h after the blood meal, mosquito midguts were incubated separately with BSA (blank control), recombinant FREP1 premixed with preimmune mouse serum, and recombinant FREP1 premixed with anti-FBG mouse serum. After removing the supernatant, the midgut-bound recombinant FREP1 was quantified by antibodies against N-FREP1 protein using ELISA.
