Figure 2.

NE suppresses CSE-induced HO-1 expression. BEAS-2B cells were pretreated with vehicle control (VC) or NE (1 units/ml) for 4 h and then stimulated with CSE (1%) for 12 (A) or 24 h (B). A, total RNA was isolated and quantitative real-time PCR for HO-1 and GAPDH was performed. Data represent the mean ± S.D. of triplicate experiments. **, p < 0.05. B, total cellular extracts were subjected to Western blot analysis for HO-1 and GAPDH. The results are representative of three independent experiments.