Figure 3.

NE does not inhibit CSE-induced nuclear translocation and DNA-binding activity of Nrf2. A and B, BEAS-2B cells were pretreated with vehicle control (VC) or NE (1 units/ml) for 4 h and then stimulated with CSE (1 or 2%) for 4 h. Nuclear and cytoplasmic proteins were extracted and subjected to Western blot analysis for Nrf2, PARP-1, KEAP1, and Hsp90 (A). Nrf2 activity using nuclear extracts was determined. Nuclear proteins from COS-7 cells transfected with Nrf2 expressing vector were used as a positive control (B). Data represent the mean ± S.D. of triplicate experiments. **, p < 0.05. The results are representative of three independent experiments. N.S., not significant.