Figure 4.

NE decreases SIRT1 expression, which mediates the reduction of HO-1 expression in CSE-treated cells. A, BEAS-2B cells were treated with vehicle control (VC) or NE (1 units/ml) for the indicated times. B, cells were transiently transfected with Myc/His-tagged SIRT1 (Myc/His-SIRT1) expression vector or control vector (Myc/His-CV). Forty-eight hours after transfection, the cells were pretreated with VC or NE (1 units/ml) for 4 h and then incubated with CSE (1%) for 24 h. Total cellular extracts were subjected to Western blot analysis for SIRT1, HO-1, and GAPDH. The results are representative of three independent experiments.