Figure 7.

Decreased level of SIRT1 in NE-treated primary HBECs and in lung homogenates from smokers and COPD patients. A, primary human bronchial epithelial cells were pretreated with vehicle control (VC) or NE (1 units/ml) for 4 h and then stimulated with CSE (1 and 2%) for 24 h. Cell lysates were subjected to Western blot analysis for SIRT1, HO-1, and GAPDH. B, lung homogenates from normal (non-smoker) (n = 6), smoker (n = 9), and COPD (n = 9) patients were subjected to Western blot analysis for SIRT1 and GAPDH. C, gel data were quantified using Scion image densitometry. Data represent the mean ± S.D. normal versus smoker: **, p = 0.0299, normal versus COPD: **, p = 0.0027.