Activation of PLA2 mediates BaP-induced inflammation in a Ca2+-dependent manner. (A) Activity of PLA2 in A549 cells. The A549 cell lines were treated with control or BaP (4 μM) for the indicated hours (n = 6). (B) Levels of IL8 in the supernatants of A549 cells. After pretreatment with PBB (100 μM), MAFP (5 μM) or VPLB (3 μM) for 1 h, the A549 cells were treated with control or BaP (4 μM) for 24 h (n = 6). (C) The mRNA levels of Pla2g2a, Pla2g4a and Pla2g10 in A549 cells. A549 cells were treated with control or BaP (4 μM) for 12 h (n = 4). (D) Intracellular Ca2+ level in A549 cells. After preloading with Fluo3-AM (3 μM) for 1 h, the A549 cells were treated with control or BaP (4 μM) for the indicated minutes (n = 16). (E) Levels of IL8 in the supernatants of A549 cells. After pretreatment with BAPTA-AM (50 μM) or EGTA (5 mM) for 1 h, the A549 cells were treated with control or BaP (4 μM) for 24 h (n = 4). All of the data are presented as the means ± SEM. (A, B, E) One-way ANOVA with Tukey's correction: * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with the BaP treatment group. (C) Two-tailed Student's t-test: ** P < 0.05 compared with the control group.