FIG 4.
Control of flagellin expression by flbTL in bacteria grown in liquid HMY medium with arabinose (Ara) or mannitol (Man) as carbon source. (A) SDS-PAGE of the B. diazoefficiens extracellular subpolar (FliC) and lateral (LafA) flagellins in the wild-type, the ΔflbTL strain, the ΔflbTL strain complemented with the plasmid pFAJ:: flbTL that carries a wild-type copy of flbTL under the direction of the nptII promoter, or the lafR::Km strain complemented with the plasmid pFAJ::flbTL or pFAJ::lafR. (B) Western blots of the cellular B. diazoefficiens proteins FliC and LafA, as visualized by an anti-lafA polyclonal antiserum, from the wild-type strain either alone or complemented with the plasmid pFAJ::flbTL or from the lafR::Km strain complemented with the plasmid pFAJ::flbTL. The polyclonal anti-lafA serum exhibited some cross-reaction against FliC, whose activity in this experiment served as an internal standard.