FIG 5.
IL-1β release by hDCs. (A) Results after incubation with M. hominis PG21 for 4 h and 24 h. ★, significantly different (P < 0.05) from IL-1β concentration released after coincubation with PBS at the same time point; ○, significantly different (P < 0.05) from IL-1β concentration released after coincubation for 4 h according to the Newman-Keuls test. (B) Results after incubation with M. hominis PG21 and its detergent fractions. (C) Results after coincubation with M. hominis PG21 with and without the caspase inhibitor YVAD-fmk. Mh, M. hominis PG21. Results are shown as the means and standard errors of the means from three independent experiments. PBS, negative control; LPS, positive control; Mh, M. hominis PG21; Mb, M. hominis PG21 membranes; APTX, TX-114 aqueous phase (nonextracted proteins); DPTX, TX-114 detergent phase (extracted proteins); IFSar, Sarkosyl-insoluble fraction (nonextracted proteins); SFSar, Sarkosyl-soluble fraction (extracted proteins); IFSar-DPTX, Sarkosyl-insoluble fraction extracted by TX-114 (detergent phase); IFSar-APTX, Sarkosyl-insoluble fraction extracted by TX-114 (aqueous phase); NS, statistically nonsignificant according to the Newman-Keuls test.