TABLE 1.

Lipoproteins identified by LC-MS/MS in eluates E2 to E8^a

Gene locus	Product	No. of peptides identified in eluate:							Calculated MM (kDa)
		E2 (100–200 kDa)	E3 (70–100 kDa)	E4 (55–70 kDa)	E5 (40–55 kDa)	E6 (30–40 kDa)	E7 (25–30 kDa)	E8 (15–25 kDa)
MHO_0280	Hypothetical protein, predicted lipoprotein		47	28	8	6			80.5
MHO_0290	Pseudogene of hypothetical protein (N-terminal part), predicted lipoprotein		55	21	11		2		67.7
MHO_0530	Lmp1 protein	13	5	2					170.7
MHO_0540	Lmp-related protein		3	28					70.0
MHO_0720	Conserved hypothetical protein, predicted lipoprotein		53	20					75.2
MHO_0730	Hypothetical protein, putative nuclease, predicted lipoprotein						2		34.3
MHO_0780	Conserved hypothetical protein, predicted lipoprotein		24	10					78.2
MHO_0790	Conserved hypothetical protein, predicted lipoprotein	5	61	27	13	2	2		78.2
MHO_1510	P100 = OppA, oligopeptide ABC transporter substrate-binding protein	44	36	24	7				105.8
MHO_1640b	Lmp3 protein	4							178.0
MHO_1730	Hypothetical protein, predicted lipoprotein						11		27.1
MHO_2080	Conserved hypothetical protein, predicted lipoprotein			8					64.3
MHO_2340	Hypothetical protein, putative adhesin, predicted lipoprotein			18					64.6
MHO_2440	Hypothetical protein, predicted lipoprotein					4	10		24.3
MHO_2620	Hypothetical protein, predicted lipoprotein			18	2				50.5
MHO_3070	Lmp related protein				24				44.2
MHO_3100	P75 related protein, predicted lipoprotein			39					62.3
MHO_3200	Conserved hypothetical protein, putative peptidase, predicted lipoprotein	5	85	47	14	11	4	10	89.6
MHO_3470	P50 = Vaa, surface lipoprotein adhesin	17	17	32	76	18	9	13	51.1
MHO_3490	P60			30					64.2
MHO_3620b	P37-like					4			41.8
MHO_3660	P120	25	6	2					119.7
MHO_3720	P75 protein precursor	4	25	65	4	2	4	5	72.4
MHO_3730	Lmp-related protein		27	2					77.8
MHO_4400	Hypothetical protein, predicted lipoprotein						3		29.7
MHO_4720	Hypothetical protein, predicted lipoprotein		2				9	7	28.3

^aUnderlined numbers correspond to the numbers of distinct peptides detected for a nonfragmented lipoprotein. The calculated molecular mass (MM) ignores the signal peptide. E2 and E6 eluates, which did not induce IL-23 production, were analyzed for reference.

^bMHO_1640 and MHO_3620 were not considered bioactive because they were present solely in the E2 and E6 eluates, which did not induce IL-23 release from hDCs.