TABLE 2.
Alignment of N-terminal parts of the 24 potential bioactive lipoproteins of M. hominis PG21 and of mycoplasmal bioactive lipopeptidesa
| Gene locus | Species | N-terminal amino acid sequence of protein | MM (kDa) | pI | GRAVY score | Acylation | Reference | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MHO_0280 | M. hominis | C | N | K | T | T | N | H | E | T | N | 1,161.2 | 6.74 | −2.070 | This study | |||||
| MHO_0290 | M. hominis | C | K | D | P | N | K | P | E | V | K | 1,157.3 | 8.18 | −1.870 | This study | |||||
| MHO_530 | M. hominis | C | G | G | L | A | I | A | T | T | A | 877.0 | 5.52 | 1.400 | This study | |||||
| MHO_0540 | M. hominis | C | R | F | C | K | K | P | N | K | Q | 1,251.5 | 9.85 | −1.700 | This study | |||||
| MHO_0720 | M. hominis | C | G | H | T | G | T | G | Y | G | F | 999.0 | 6.73 | −0.220 | This study | |||||
| MHO_0730 | M. hominis | C | K | T | V | N | K | E | K | I | N | 1,176.4 | 9.20 | −1.170 | This study | |||||
| MHO_0780 | M. hominis | C | S | T | T | E | N | S | K | Y | G | 1,089.1 | 5.99 | −1.310 | This study | |||||
| MHO_0790 | M. hominis | C | S | T | T | E | N | S | K | Y | G | 1,089.1 | 5.99 | −1.310 | This study | |||||
| MHO_1510 | M. hominis | C | K | I | D | P | A | Y | E | K | G | 1,123.3 | 6.06 | −0.930 | This study | |||||
| MHO_1730 | M. hominis | C | N | D | P | K | N | K | K | N | P | 1,157.3 | 9.20 | −2.640 | This study | |||||
| MHO_2080 | M. hominis | C | A | I | L | P | V | S | C | S | L | 1,005.2 | 5.51 | 1.990 | This study | |||||
| MHO_2340 | M. hominis | C | I | K | T | K | K | P | E | V | K | 1,173.4 | 9.63 | −1.020 | This study | |||||
| MHO_2440 | M. hominis | C | K | T | T | N | D | S | Q | N | S | 1,097.1 | 5.83 | −1.840 | This study | |||||
| MHO_2620 | M. hominis | C | V | K | T | K | K | P | E | G | E | 1,118.3 | 8.18 | −1.470 | This study | |||||
| MHO_3070 | M. hominis | C | N | N | K | N | S | K | L | T | K | 119.3 | 9.79 | −1.740 | This study | |||||
| MHO_3100 | M. hominis | C | S | N | K | Q | D | K | K | E | D | 1,194.2 | 6.11 | −2.750 | This study | |||||
| MHO_3200 | M. hominis | C | K | N | T | N | K | K | T | N | N | 1,164.3 | 9.79 | −2.460 | This study | |||||
| MHO_3470 | M. hominis | C | N | D | D | K | L | A | E | K | N | 1,149.2 | 4.56 | −1.720 | This study | |||||
| MHO_3490 | M. hominis | C | K | K | E | K | E | D | S | Q | Q | 1,222.3 | 6.17 | −2.750 | This study | |||||
| MHO_3660 | M. hominis | C | T | H | T | N | N | D | E | L | N | 1,160.1 | 4.35 | −1.580 | This study | |||||
| MHO_3720 | M. hominis | C | K | N | E | K | S | N | A | E | Y | 1,185.2 | 6.13 | −1.960 | This study | |||||
| MHO_3730 | M. hominis | C | T | V | T | V | K | V | K | E | K | 1,134.4 | 9.20 | −0.150 | This study | |||||
| MHO_4400 | M. hominis | C | N | K | T | A | T | I | T | L | N | 1,078.2 | 8.22 | −0.040 | This study | |||||
| MHO_4720 | M. hominis | C | G | G | E | K | F | N | A | F | A | 1,043.1 | 5.99 | 0.000 | This study | |||||
| MALP-2 | M. fermentans | C | G | N | N | D | E | S | N | I | S | F | K | E | K | 2,134.1 | 4.68 | −1.500 | Pam2 | 36 |
| FSL-1 | M. salivarium | C | G | D | P | K | H | P | K | S | F | 1,666.1 | 8.21 | −1.360 | Pam2 | 12 | ||||
| MPPL-1 | M. pneumoniae | C | T | G | I | Q | A | D | L | R | N | L | I | K | 1,341.5 | 8.41 | −0.133 | Pam2 | 13 | |
| P2C-RGDS | Mycobacterium tuberculosis | C | R | G | D | S | 1,087.5 | 5.83 | −1.34 | Pam2 | 25 | |||||||||
| PGTP2-RL | Porphyromonas gingivalis | C | N | S | Q | A | K | 649.7 | 8.22 | −1.233 | Pam3 | 37 | ||||||||
| Murein LPP | E. coli | C | S | S | N | K | I | D | E | L | S | D | D | 1,325.3 | 3.84 | −1.083 | Pam3 | 21 | ||
| MDPL | M. synoviae | C | G | D | Q | T | P | A | P | E | P | T | P | G | N | 1,383 | 3.67 | −1.307 | Pam2 | 38 |
| DSL-1 | M. hominis | C | G | G | E | K | F | N | 753.8 | 5.99 | −0.914 | Pam2 | This study | |||||||
a
Molecular mass (MM), isoelectric point (pI), and GRAVY score were determined using ProtParam software (http://web.expasy.org/cgi-bin/protparam/protparam). Hydrophobic amino acids are in boldface. Pam, palmitic acid; Pam2, diacylated lipoproteins with two palmitic acids; Pam3, triacylated lipoproteins with three palmitic acids.